Contact an Account ManagerIsolation and separation of the glycan strands from murein of E. coli by reversed phase HPLC
Summary
Type: HPLC
Application #: 102730
Catalog#: 720065.46 - MN HPLC Column, Nucleosil C18, 300A, 5um, 4.6x250mm
Detection: upper trace UV, 202nm, lower trace radioactivity of 31µl aliquots of 125µl fractions
Conditions: temperature 50°C
eluent: convex gradient from 5 to 11% acetonitrile in 100mM Na phosphate buffer pH 2 over 90min
flow rate 0.5ml/min
Authors: Harz H
Source: Anal Biochem 190 (1990) 120 - 128
Details
Legend: Fig. 2. Separation of amidase digest of 150µg 3HGlcNAc-murein of E. coli EH
3247, numbers above peaks indicate length in disaccharide units.